Part:BBa_K2774001:Design

tsRNA

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BamHI site found at 201
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

Toxic small RNAs are small non-coding RNA. We use the tsRNA isolated from Burkholderia cenocepacia. Current study showed that it is toxic to the E. coli. host and reproducibly inhibit E. coli growth. TsRNA harbored a sequence that was complementary to the consensus ribosomal binding site (RBS). It is hypothesized that it might target target and misregulate essential genes within the E. coli cell. TsRNA cannot penetrate the bacterial cell from the outside, so it is unable to affect bacteria except E. coli. host cells. Additionally, producing tsRNA doesn’t require translational process, thus the respond time is more rapid. As a result, tsRNA become an ideal suicide substance for our design.

Source

Burkholderia cenocepacia

References

Kimelman, A.; Levy, A.; Sberro, H.; Kidron, S.; Leavitt, A.; Amitai, G.; Yoder-Himes, D. R.; Wurtzel, O.; Zhu, Y.; Rubin, E. M.; Sorek, R., A vast collection of microbial genes that are toxic to bacteria. Genome research 2012, 22 (4), 802-9.